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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Facts Cyclin E belongs towards the remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add for the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities as being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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